For virtually half in the homoplasia. SNP s1319 in Rv0280 (a PPE household protein) happens in seven positions inside the MST. Rv2082, annotated as hypothetical protein in H37Rv refseq NC_000962.2 contains 5 homoplasic SNPs accounting for 14 out from the 81 excess. The red star within the central position of the Figure three MST indicates the approximate position of the MRCA for the MTBC complicated, according to Namouchi et al. , i.e. the branching point of your ``M. canettii'' taxon, under the hypothesis that ``M. journal.pone.0133807
canettii'' can indeed be deemed as an outgroup. Three branches had been observed to radiate from this red star; one corresponds to lineage 1 like EAI (pink), a second a single leads to lineages 5, six as well as the animal lineage including M. bovis (brown, green, orange), as well as the third a single leads to lineages 2, 3 and four (blue, purple, red). Lineages 2? and lineage four split at a distance of 241 SNPs from the red star. The secondary split in between lineages two and 3 occurs very shortly afterwards, 42 SNPs later. The distance in the red star towards the tip from the branches is closely related inside each and every lineage and across lineages: it is actually roughly 700?00 SNPs extended for lineages 1 to five, a little bit more than 900 SNPs for lineage 6 and 7, as well as a tiny extra than 1000 SNPs in the animal lineage (Table 1). The Percy644 control strain fits in the lineage two (including the Beijing clade) as expected. It contributes to 1.five of the size of the MST (202 bp terminal branch). pnas.1222674110
Similarly, Percy717, Percy34 and Percy119 belong to their predicted lineages, five, 6 and six respectively and they contribute to eight.four with the total MST. Interestingly these two groups of strains define two new deep branching nodes and considerably older MRCAs within these lineages (indicated by blue stars in Figure 3). These nodes are positioned approximately midway along the lengthy initial lineage 5 and lineage six branches. Percy209 contributes a 354 SNPs terminal branch corresponding to two.six in the MST. Percy209 from Mali and M4100A from South Korea each showing a Manu ancestor SIT523 spoligotype split simultaneously from the rest of lineage two top towards the Beijing sublineage. As initially recommended by MLVA and spoligotyping, Percy256 and Percy556 are very exceptional in comparison with each of the other studied genomes. They introduce a 876 SNPs long terminal branch towards the tree, which contributes to six.5 from the MST. Thisremarkable branch splits in the branch leading to lineages 2, three, and 4 only 66 SNPs away in the red star. Prior investigations identified seven interruptions of coding sequences (ICDS), numbered ICDS0013, 0037, 0038, 0045, 0055, 0073, 0078, which occurred along superlineage 2-3-4 prior to the split involving lineage 4 and lineages two? . ICDS0045 couldn't be analysed in Percy256 and Percy556, whereas ICDS0013 (Rv0618-Rv0619) and ICDS0038 (Rv1549-Rv1550) are present in Percy256 and Percy556. The 4 other folks are still Ues quantify how completers were considerably additional probably to possess a
intact in each strains. We propose to call this
age, lineage 7. Percy256 and Percy556 isolated respectively in 1998 and 2007, are separated from each other by only eight SNPs.Rooting the MTBCLineage 7 is unique by becoming the only lineage using a geographic location apparently restricted to the Horn of Africa and as such coincident with all the ``M.